R&D Systems™ Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag Protein

Catalog No.	Quantity
AVI720050	50 μg

Catalog No. AVI720050 Supplier R&D Systems™ Supplier No. AVI720050

Description

Biotinylated

• Measured by its binding ability in a functional ELISA.
• When Human ICAM-1 (14C11) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ICAM-1/CD54 Fc Chimera Avi-tag binds with an ED50 of 3 to 18 ng/mL.
• Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells.
• The ED50 for this effect is 0.3 to 2.7 μg/mL.

Specifications

• Accession Number: P05362
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3383
• Molecular Weight (g/mol): 78 kDa
• Name: BB2, CD54, CD54 antigen, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM1, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor, P3.58
• Quantity: 50 μg
• Source:

  Human embryonic kidney cell, HEK293-derived human ICAM-1/CD54 protein

  Human ICAM-1 (Gln28-Glu480) Accession # P05362IEGRMDHuman IgG1 (Pro100-Lys330)GGAvi-tag

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Biotin
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.