R&D Systems™ Recombinant Human ICOS Fc Chimera Protein

Catalog No.	Quantity
169CS050	50 μg

Catalog No. 169CS050 Supplier R&D Systems™ Supplier No. 169CS050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 1-4 μg/mL in the presence of 3 μg/mL Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7 ) and at least 20 ng/mL of anti-CD3. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: Q9Y6W8
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 29851
• Molecular Weight (g/mol): M.W. (Observed): 50-60 kDa, reducing conditions; M.W. (theoretical): 40.3 kDa (monomer)
• Name: ICOS Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human ICOS protein Human ICOS (Glu21-Phe141) (N-terminus) Accession # Q9Y6W8 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: ICOS
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: ICOS