R&D Systems™ Recombinant Human IFN-beta Protein

Catalog No. 84-99I-F010

Supplier: R&D Systems™ 8499IF010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human IFN-beta Protein is derived from CHO. The Recombinant Human IFN-beta Protein has been validated for the following applications: Bioactivity.

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Specifications

• Accession Number: P01574
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
• Gene ID (Entrez): 3456
• Molecular Weight (g/mol): Observed M.W.: 20-26kDa, reducing conditions, Theoretical M.W.: 20kDa
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human IFN-beta protein Met22-Asn187
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: Fibroblast interferon, IFB, IFBIFNB, IFF, IFNB, IFNB1, IFNbeta, IFN-beta, interferon beta, interferon, beta 1, fibroblast, MGC96956
• Common Name: IFN-beta
• Gene Symbol: IFNB1
• Biological Activity: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is typically 5-30 pg/mL. The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN-beta WHO International Standard (NIBSC code: 00/572).
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Met22-Asn187
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining