R&D Systems™ Recombinant Human IFN-gamma Biotinylated Protein

Catalog No.	Quantity
BT285050	50 μg

Catalog No. BT285050 Supplier R&D Systems™ Supplier No. BT285050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human IFN-gamma R1/CD119 (Catalog # 673-IR/CF ) is immobilized at 3μg/mL (100μL/well), Biotinylated Recombinant Human IFN-gamma (Catalog # BT285) binds with an ED50 of 3.00-27.0 ng/mL.

Specifications

• Accession Number: CAA31639.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2μm filtered solution in Sodium Succinate, Mannitol and Tween 80.
• Molecular Weight (g/mol): M.W. (Observed): 14-20 kDa, under reducing conditions., M.W. (Theoretical): 16.9 kDa
• Quantity: 50 μg
• Source: E. coli-derived human IFN-gamma protein Gln24-Gln166, with a N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Conjugate: Biotin
• Species: Human
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile, deionized water.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.