R&D Systems™ Recombinant Human IGF-I/IGF-1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL.

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Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS.
• Gene ID (Entrez): 3479
• Molecular Weight (g/mol): 7.6 kDa
• Name: IGF-I/IGF-1
• Quantity: 200 μg
• Source: E. coli-derived human IGF-I/IGF-1 protein Gly49-Ala118
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.