R&D Systems™ Recombinant Human IL-10, Animal Free Protein

Description

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.0750-0.750 ng/mL.

The specific activity of recombinant human IL-10 is >2.50 x 106 units/mg, which is calibrated against the human IL-10 WHO Reference Reagent (NIBSC code: 93/722).

Specifications

• Accession Number: P22301.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3586
• Molecular Weight (g/mol): M.W.-observed: 18 kDa, reducing conditions, M.W.-theroretical: 19 kDa
• Quantity: 1 mg
• Source: E. coli-derived human IL-10 protein Ser19-Asn178, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10 mgC126451, interleukin 10, interleukin-10, TGIF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-10