R&D Systems™ Recombinant Human IL-10 GMP Protein, CF

Catalog No.	Quantity
10-64G-MP050	50 μg

Catalog No. 10-64G-MP050 Supplier R&D Systems™ Supplier No. 1064GMP050

Description

Animal Free

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.0750-0.750 ng/mL.

The specific activity of recombinant human IL-10 is >2.50 x 106 units/mg, which is calibrated against the human IL-10 WHO Reference Reagent (NIBSC code: 93/722).

Specifications

• Accession Number: P22301.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3586
• Molecular Weight (g/mol): Observed: 18 kDa, reducing conditions, MolecularWeight-theroretical: 19 kDa
• Quantity: 50 μg
• Source: E. coli-derived human IL-10 protein Ser19-Asn178, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Regulatory Status: GMP
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
• Gene Symbol: IL10
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 18762 Da ± 20 Da
• Protein: IL-10