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R&D Systems™ Recombinant Human IL-10 Protein

Catalog No. 1064ILB250
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1 mg
10 μg
250 μg
50 μg
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Catalog No. Quantity
1064ILB250 250 μg
1064ILB010 10 μg
1064ILB01M 1 mg
1064ILB050 50 μg
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Description

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.075-0.750 ng/mL.

Specifications

Accession Number P22301.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 3586
Molecular Weight (g/mol) M.W.-observed: 18 kDa, under reducing conditions., M.W.-theroretical: 19 kDa
Quantity 250 μg
Source E. coli-derived human IL-10 protein Ser19-Asn178, with a N-terminal Met
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10 mgC126451, interleukin 10, interleukin-10, TGIF
Conjugate Unconjugated
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein IL-10
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