R&D Systems™ Recombinant Human IL-10 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.075-0.750 ng/mL.

Specifications

• Accession Number: P22301
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 3586
• Molecular Weight (g/mol): 18.6 kDa
• Quantity: 250 μg
• Source: <EM>Spodoptera frμgiperda</EM>, <EM>Sf</EM> 21 (baculovirus)-derived human IL-10 protein Ser19-Asn178
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
• Gene Symbol: IL10
• Conjugate: Unconjugated
• Cross Reactivity: IL-10
• Species: Human
• Reconstitution: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.