R&D Systems™ Recombinant Human IL-12 Biotinylated Protein

Description

Biotinylated, Linked Heterodimer, GS Linker, Defined heterodimer stoichiometry.

Measured by its binding ability in a functional ELISA. Whe Recombinant Human IL-12 R beta 1 Fc Chimera Protein (Catalog # 839-B1 ) is immobilized at 1.00 μg/mL (100uL/well), Biotinylated Recombinant Human IL-12 (Catalog # BT10018) binds with an ED50 of 40.0-400 ng/mL.

Specifications

• Accession Number: P29460.1, P29459.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W.-observed: 68-80 kDa, under reducing conditions., M.W.-theroretical: 59 kDa
• Quantity: 25 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-12 protein Human IL-12p40 (Ile23-Ser328) (C-terminus) Accession # P29460.1 GSGSSRGGSGSGGSGGGGSK Human IL-12p35 (Arg23-Ser219) (C-terminus) Accession # P29459.2 (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.