R&D Systems™ Recombinant Human IL-12 (linked heterodimer) Protein

Description

GS Linker, Defined heterodimer stoichiometry.

• Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts
• ED50 for this effect is 0.01 to 0.05 ng/mL

Specifications

• Accession Number: P29460.1 (IL-12p40), P29459.2 (IL-12p35)
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3592
• Molecular Weight (g/mol): Observed M.W.: 63-75 kDa, under reducing conditions.
• Name: CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
• Quantity: 100 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-12 protein Human IL-12p40 (Ile23-Ser328) (N-terminus) Accession # P29460.1 GSGSSRGGSGSGGSGGGGSK Human IL-12p35 (Arg23-Ser219) (N-terminus) Accession # P29459.2(C-terminus)
• Storage Requirements: 12 months from date of receipt, ≤-20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤-20 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100-200 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.