R&D Systems™ Recombinant Human IL-12 p35 (C96S) Protein

Catalog No.	Quantity
11209IL050	50 μg

Catalog No. 11209IL050 Supplier R&D Systems™ Supplier No. 11209IL050

Description

Measured by its ability to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells, the concentration of Recombinant Human IL-12p35 (C96S) Monomer (Catalog # 11209-IL) that produces 50% of the response is 0.300-3.00 ng/mL when Recombinant Human IL-12p40 Monomer (Catalog # 309-IL ) is present at 0.25 μg/mL.

Specifications

• Accession Number: P29459.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3592
• Molecular Weight (g/mol): M.W.-observed: 28-37 kDa, under reducing conditions., M.W.-theroretical: 23 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-12 protein Arg23-Ser219 (Cys96Ser)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12 A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-12