R&D Systems™ Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera

Catalog No.	Quantity
118AP100	100 μg

Catalog No. 118AP100 Supplier R&D Systems™ Supplier No. 118AP100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR ). The ED50 for this effect is 2-6 μg/mL in the presence of 30 μg/mL of soluble IL-18 R alpha/Fc Chimera and 40 ng/mL of human IL-18.

Specifications

• Accession Number: O95256
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 8807
• Molecular Weight (g/mol): M.W. (Observed): 78-93 kDa, reducing conditions; M.W. (theoretical): 65.1 kDa (monomer)
• Name: IL-18 R beta/IL-1 R7 Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived human IL-18 R beta/IL-1 R7 protein Human IL-18 R beta (Phe20 - Arg356) (N-terminus) Accession # O95256 DIEGRMD Human IgG1 (Pro100 - Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: IL18RAP
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: IL-18 R beta/IL-1 R7/ACPL