R&D Systems™ Recombinant Human IL-2 Protein, Animal-Free

Catalog No.	Quantity
RD10000268	1 mg
RD10000267	50 μg

Catalog No. RD10000268 Supplier R&D Systems™ Supplier No. BT002AFL01M/LQ

Description

Contains the Cysteine to Serine substitution found in Proleukin™ (aldesleukin)

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL. The specific activity of recombinant human IL-2 is >5.00 x 106 IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).

Specifications

• Accession Number: P60568.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in Sodium Acetate.
• Gene ID (Entrez): 3558
• Molecular Weight (g/mol): M.W.-observed: 13 kDa, under reducing conditions., M.W.-theroretical: 15.5 kDa
• Quantity: 1 mg
• Source: E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and without a N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, ≤ -65 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.01 EU per 1 μg of the protein by the LAL method.
• Gene Alias: aldesleukin, IL2, IL-2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, Proleukin, T cell growth factor, T-cell growth factor, TCGF
• Conjugate: Unconjugated
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-2