R&D Systems™ Recombinant Human IL-21 Biotinylated Protein

Description

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-21R Fc Chimera Protein (Catalog # 991-R2 ) is immobilized at 0.500 μg/mL, 100 μL/well, Biotinylated Recombinant Human IL-21 (Catalog # BT8879) binds with an ED50 of 1.50-9.00 ng/mL.

Specifications

• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 59067
• Molecular Weight (g/mol): M.W.-observed: 15-20 kDa, under reducing conditions., M.W.-theroretical: 15 kDa
• Quantity: 25 μg
• Source: E. coli-derived human IL-21 protein Gln32-Ser162
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: IL-21 Za11interleukin-21, interleukin 21, interleukin-21 isoform
• Conjugate: Biotin
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-21