R&D Systems™ Recombinant Human IL-21 (HEK293 Expressed) Protein

Description

Carrier Free Recombinant Protein

Measured in a cell proliferation assay using B9 mouse hybridoma cells. The ED50 for this effect is 3.00-30.0 ng/mL.

Specifications

• Accession Number: Q9HBE4.3
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 59067
• Molecular Weight (g/mol): MolecularWeight-observed: 15-17 kDa, under reducing conditions., MolecularWeight-theroretical: 15 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-21 protein Gln32-Ser162
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CVID11, IL21, IL-21 Za11interleukin-21, interleukin 21, interleukin-21 isoform, Za11
• Conjugate: Unconjugated
• Reconstitution: Reconstitute 10 μg size at 100 μg/mL and other sizes (at 500 μg/mL in PBS.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-21