R&D Systems™ Recombinant Human IL-23 (HEK293 Expressed) Protein

Description

Carrier Free Recombinant Protein

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.100-2.00 ng/mL.

Specifications

• Accession Number: P29460.1 & AAG37232.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 51561
• Molecular Weight (g/mol): MolecularWeight-observed: 57-71 kDa, under reducing conditions., MolecularWeight-theroretical: 55 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-23 protein Human IL-12p40 (Ile23-Ser328) (N-terminus) Accession # P29460.1 GSGSSRGGSGSGGSGGGGSKL Human IL-23p19 (Arg20-Pro189) Accession # AAG37232.1 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: IL23, IL-23, IL-23 p19/IL-12 p40, IL-23 A, IL-23-A, IL-23p19, IL-23p19/IL-12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T-cell activation, MGC79388, SGRF, SGRFIL-23 subunit alpha
• Conjugate: Unconjugated
• Reconstitution: Reconstitute 10 μg size at 100 μg/mL in PBS. Reconstitute all the other size (at 500 μg/mL in PBS.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-23 A/IL-23 P19