R&D Systems™ Recombinant Human IL-27 Ra/WSX-1/TCCR Avi His Protein

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-27 (Catalog # 2526-IL/CF ) is immobilized at 4 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-27 R alpha/WSX-1/TCCR Avi-tag His-tag (Catalog # AVI11270) binds with an ED50 of 30.0-360 ng/mL.

Specifications

• Accession Number: Q6UWB1.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W. Observed: 81-92 kDa, under reducing conditions., M.W. Theoretical: 57 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-27 R alpha/WSX-1/TCCR protein Human IL-27 R alpha/WSX-1/TCCR (Gln33-Lys516) (N-terminus) Accession # Q6UWB1.2 Avi-tag 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.