R&D Systems™ Recombinant Human IL-33 Biotinylated Protein

Catalog No.	Quantity
BT3625025	25 μg

Catalog No. BT3625025 Supplier R&D Systems™ Supplier No. BT3625025

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human ST2/IL-33R Fc Chimera (Catalog # 523-ST ) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-33 (Catalog # BT3625) binds with an ED50 of 0.900-9.00 ng/mL.

Specifications

• Accession Number: O95760.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with Trehalose.
• Gene ID (Entrez): 90865
• Molecular Weight (g/mol): M.W.-observed: 18-22 kDa, under reducing conditions., M.W.-theroretical: 18 kDa
• Quantity: 25 μg
• Source: E. coli-derived human IL-33 protein Ser112-Thr270
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL1F11, IL-1F11, IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin-33, NFHEV, NF-HEV, NF-HEVNFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-33