R&D Systems™ Recombinant Human IL-4 Protein

Description

Carrier Free Recombinant Protein

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.0400-0.320 ng/mL.

Specifications

• Accession Number: P05112.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3565
• Molecular Weight (g/mol): M.W. (Observed): 13 kDa, under reducing conditions., M.W. (Theoretical): 15.1 kDa
• Quantity: 100 μg
• Source: E. coli-derived human IL-4 protein His25-Ser153, with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4B_cell stimulatory factor 1, IL4E12, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute the 10μg size at 100μg/mL in PBS. Reconstitute all other sizes at 500μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-4