R&D Systems™ Recombinant Human IL-4 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.

Specifications

• Accession Number: P05112
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 3565
• Molecular Weight (g/mol): 15 kDa
• Quantity: 20 μg
• Source: <EM>E. coli</EM>-derived human IL-4 protein His25-Ser153, with an N-terminal Met
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4B_cell stimulatory factor 1, IL4E12, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
• Gene Symbol: IL4
• Conjugate: Unconjugated
• Cross Reactivity: IL-4
• Species: Human
• Reconstitution: Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.