R&D Systems™ Recombinant Human IL-7 GMP Protein, CF

Catalog No.	Quantity
BT007GMP010	10 μg
BT007GMP025	25 μg

Catalog No. BT007GMP010 Supplier R&D Systems™ Supplier No. BT007GMP010

Description

Animal-Free.

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.100-0.500 ng/mL.The specific activity of Recombinant Human IL-7 is >1.00 x 108 units/mg, which is calibrated against the human IL-7 reference standard (NIBSC code: 90/530).

Specifications

• Accession Number: P13232.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3574
• Molecular Weight (g/mol): Observed: 17 kDa, under reducing conditions, Observed: 17 kDa
• Quantity: 10 μg
• Source: E. coli-derived human IL-7 protein Asp26-His177, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Regulatory Status: GMP
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: IL7, IL-7interleukin-7, interleukin 7, Lymphopoietin-1, PBGF
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 17507 Da ± 50 Da
• Protein: IL-7