R&D Systems™ Recombinant Human IL-7 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 3574
• Molecular Weight (g/mol): 17 kDa
• Name: IL-7
• Quantity: 25 μg
• Source: E. coli-derived human IL-7 protein Asp26-His177,with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU per 1μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.