R&D Systems™ Recombinant Human Insulin R/CD220 (aa 28-956) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Insulin R/CD220 (aa 28-956) Protein is derived from HEK293. The Recombinant Human Insulin R/CD220 (aa 28-956) Protein has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3643
• Molecular Weight (g/mol): Observed M.W.: 105-120kDa ( alpha subunit) and 37-49kDa ( beta subunit), reducing conditions, Theoretical M.W.: 107kDa ( alpha & beta heterodimer)
• Quantity: 50 μg
• Source: Human embryonic kidney cell,HEK293-derived human Insulin R/CD220 protein His28-Arg762( alpha subunit) and Ser763-Lys956 with a c-terminal 10-His tag ( beta subunit)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: CD 220, CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, INSR, insulin receptor, InsulinR, IR
• Common Name: Insulin R/CD220
• Gene Symbol: INSR
• Biological Activity: Measured by its binding ability in a functional ELISA. When 15ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220 B Isoform, the concentration of Recombinant Human Insulin R/CD220 B Isoform that produces 50% of the optimal binding response is typically 0.1-0.5μg/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Human Insulin R alpha (His28-Arg762) Accession # P06213, N-terminus; Human Insulin R beta (Ser763-Lys956) Accession # P06213; HHHHHHHHHH, C-terminus
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining