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R&D Systems™ Recombinant Human KEAP1/CUL3/NEDD8/RBX1 Complex Protein, CF

Catalog No. E3701025
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25 μg
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Catalog No. Quantity
E3701025 25 μg
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Description

His10

Typical protein concentration for use in vitro will depend on experimental conditions. For in vitro ubiquitination reactions, we suggest an initial KEAP1/CUL3(NEDD8)/RBX1 concentration between 10-50 nM.

Specifications

Accession Number Q14145, Q13618, Q15843, P62877
For Use With (Application) Bioactivity
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Molecular Weight (g/mol) M.W.-theroretical: 70 kDa (KEAP1), 101 kDa (neddylated CUL3), 12 kDa (RB x 1)
Quantity 25 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived human KEAP1/CUL3/NEDD8/RBX1 protein
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 48 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Conjugate Unconjugated
Purity or Quality Grade >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
Protein KEAP1/CUL3/NEDD8/RBX1
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