R&D Systems™ Recombinant Human KGF/FGF-7 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL.

Specifications

• Accession Number: P21781
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS, Na 2 SO 4 and EDTA with BSA as a carrier protein.
• Gene ID (Entrez): 2252
• Molecular Weight (g/mol): 19kDa
• Quantity: 1 mg
• Source: E. coli-derived human KGF/FGF-7 protein Cys32-Thr194,with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10EU per 1μg of the protein by the LAL method.
• Gene Alias: FGF7, FGF-7, fibroblast growth factor 7HBGF-7, HBGF7, HBGF-7, Heparin-binding growth factor 7, keratinocyte growth factor, KGFfibroblast growth factor 7 (keratinocyte growth factor)
• Gene Symbol: FGF7
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.