R&D Systems™ Recombinant Human KGF/FGF-7 Protein

Catalog No. 11-340-KG500

Supplier: R&D Systems™ 11340KG500

Description

Carrier Free Recombinant Protein

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 25.0-250 ng/mL.

Specifications

• Accession Number: P21781.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
• Gene ID (Entrez): 2252
• Molecular Weight (g/mol): MolecularWeight-observed: 22-28 kDa, under reducing conditions., MolecularWeight-theroretical: 19 kDa
• Quantity: 500 μg
• Source: Human embryonic kidney cell, HEK293-derived human KGF/FGF-7 protein Cys32-Thr194
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: FGF7, FGF-7, fibroblast growth factor 7 HBGF-7, HBGF7, HBGF-7, Heparin-binding growth factor 7, keratinocyte growth factor, KGFfibroblast growth factor 7 (keratinocyte growth factor)
• Conjugate: Unconjugated
• Reconstitution: Reconstitute 10 μg size at 100 μg/mL and all the other sizes (at 250 μg/mL in PBS.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: KGF/FGF-7