R&D Systems™ Recombinant Human Klotho beta Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When 2 μg/mL (100 μL/well) of Recombinant Human (rh) Klotho beta was immobilized onto a Ms x hKlotho beta coated plate, the concentration of rhFGF-21 that produces 50% of the optimal binding response was found to be approximately 3-18 ng/mL.

Specifications

• Accession Number: Q86Z14
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
• Gene ID (Entrez): 152831
• Molecular Weight (g/mol): M.W. (Observed): 113 - 125 kDa, reducing conditions; M.W. (theoretical): 110 kDa
• Name: Klotho beta
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Klotho beta protein Phe53-Leu997, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: KLB
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Klotho beta