R&D Systems™ Recombinant Human Klotho His-tag Protein

Catalog No.	Quantity
10-308-KL050	50 μg

Catalog No. 10-308-KL050 Supplier R&D Systems™ Supplier No. 10308KL050

Description

Mucin Stalk Chimera

Measured by its binding ability in a functional ELISA. When Recombinant Human FGF-23 (Catalog # 2604-FG ) is immobilized at 5 μg/mL (100 μL/well), Recombinant Human Klotho Mucin Stalk Chimera His-tag (Catalog # 10308-KL) binds with an ED50 of 0.2-1.8 μg/mL.

Specifications

• Accession Number: Q9UEF7 (Klotho) & P78423 (Fractalkine Mucin-like Stalk)
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 9365
• Molecular Weight (g/mol): M.W. (Observed): 110-140 kDa, under reducing conditions; M.W. (theoretical): 84 kDa
• Name: EC 3.2.1, EC 3.2.1.31, KL, klotho
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Klotho protein Human Klotho (Glu34-His549) (N-terminus) Accession # Q9UEF7 Human Fractalkine Mucin-like Stalk (Phe103-Thr338) (N-terminus) Accession # P78423 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Klotho