R&D Systems™ Recombinant Human Lactate Dehydrogenase B/LDHB Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to reduce pyruvate to lactate. The specific activity is >60,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P07195
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol.
• Gene ID (Entrez): 3945
• Molecular Weight (g/mol): M.W. (Observed): 36 kDa, reducing conditions; M.W. (theoretical): 37 kDa
• Quantity: 50 μg
• Source: E. coli-derived human Lactate Dehydrogenase B/LDHB protein Ala2-Leu334, with N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: EC 1.1.1, EC 1.1.1.27, Epididymis Secretory Protein Li 281, HEL-S-281, lactate dehydrogenase B, LDH heart subunit, LDHB, LDH-B, LDHBD, LDH-H, L-lactate dehydrogenase B chain, Renal Carcinoma Antigen NY-REN-46, TRG-5
• Common Name: Lactate Dehydrogenase B
• Biological Activity: Measured by its ability to reduce pyruvate to lactate. The specific activity is >60,000 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Ala2-Leu334, with N-terminal Met and 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Lactate Dehydrogenase B