R&D Systems™ Recombinant Human Legumain/Asparaginyl Endopeptidase

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >250 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: AAH03061.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Gene ID (Entrez): 5641
• Molecular Weight (g/mol): M.W. (Observed): 60 kDa, reducing conditions; M.W. (theoretical): 49 kDa
• Name: Legumain/Asparaginyl Endopeptidase
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human Legumain/Asparaginyl Endopeptidase protein Ile18-Tyr433, with an N-terminal 7-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Structural Form: Pro form
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: LGMN
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Legumain/Asparaginyl Endopeptidase