R&D Systems™ Recombinant Human LH alpha/beta Heterodimer Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human LH alpha/beta Heterodimer Protein is derived from CHO. The Recombinant Human LH alpha/beta Heterodimer Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P01215 ( alpha chain) & P01229 ( beta chain)
• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Observed M.W.: 15-26kDa, reducing conditions, Theoretical M.W.: 10kDa ( alpha chain), 13kDa ( beta chain)
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human LH alpha/beta Heterodimer protein Ala25-Ser116 ( alpha chain) & Ser21-Leu141 ( beta chain)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: LH alpha/beta Heterodimer
• Biological Activity: Measured by its ability to induce cAMP accumulation in MLTC-1 mouse Leydig tumor cells. Rebois, R.V. et al. (1982) J. Cell Biol. 94:70. The ED50 for this effect is typically 0.25-2.5ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Ala25-Ser116 ( alpha chain) & Ser21-Leu141 ( beta chain)
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining