R&D Systems™ Recombinant Human M-CSF (CHO-expressed) Protein

Description

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. ED50 for this effect is 0.6 to 2.4 ng/mL

Specifications

• Accession Number: P09603
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 1435
• Molecular Weight (g/mol): 25.1 kDa (monomer)
• Name: colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human M-CSF protein Glu33-Arg255
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Gene Symbol: CSF1
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.