R&D Systems™ Recombinant Human M-CSF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5-1.5 ng/mL.

Specifications

• Accession Number: NP_757350
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 1435
• Molecular Weight (g/mol): 18.5 kDa (monomer)
• Quantity: 100 μg
• Source: <EM>E. coli</EM>-derived human M-CSF protein Glu33-Ser190, with an N-terminal Met
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo., 2 to 8°C under sterile conditions after reconstitution; 3 mo., -20 to -70°C under sterile conditions after reconstitution
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU per 1 μg of the protein by the LAL method.
• Gene Symbol: CSF1
• Conjugate: Unconjugated
• Cross Reactivity: M-CSF
• Species: Human
• Reconstitution: Reconstitute at 50-500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.