R&D Systems™ Recombinant Human M-CSF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5-1.5 ng/mL.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 1435
• Molecular Weight (g/mol): 18.5 kDa (monomer)
• Name: M-CSF
• Quantity: 5 μg
• Source: E. coli-derived human M-CSF protein Glu33-Ser190,with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 50-500μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: 97%, by SDS-PAGE with silver staining.