R&D Systems™ Recombinant Human Mer His-tag Avi-tag Protein

Catalog No.	Quantity
AVI10515050	50 μg

Catalog No. AVI10515050 Supplier R&D Systems™ Supplier No. AVI10515050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human Mer His-tag Avi-tag (Catalog# AVI10515) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004 ) at 2.00 μg/mL (100 μL/well). Recombinant Human Gas6 Protein (Catalog # 885-GSB ) binds with an ED50 of 80.0-800 ng/mL.

Specifications

• Accession Number: Q12866.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W.-observed: 108-121 kDa, under reducing conditions., M.W.-theroretical: 55 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Mer protein Human Mer (Arg20-Asp500) (C-terminus) Accession # Q12866.2 6-His tag Avi-tag (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.