R&D Systems™ Recombinant Human MMP-12 Protein

Catalog No. 91-7MP-B020

Supplier: R&D Systems™ 917MPB020

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001 ). The specific activity is >500 pmol/min/μg, as measured under the described conditions.

Order Info

Ships at ambient temperature.

Specifications

• Accession Number: P39900
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, CHAPS, ZnSO4 and PEG.
• Gene ID (Entrez): 4321
• Molecular Weight (g/mol): M.W. (Observed): 53-60 kDa, reducing conditions; M.W. (theoretical): 52 kDa
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human MMP-12 protein Leu17-Cys470, with Val97Leu substitution
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: EC 3.4.24, hME, HMEEC 3.4.24.65
• Biological Activity: >500pmol/min/μg
• Product Type: Recombinant protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 0.25 mg/mL in 10 mM MES, 0.1 M NaCl, 100 μM CaCl2, 0.1% CHAPS, 1 μM ZnSO4 and 0.1% PEG., pH 6.0.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: MMP-12

For Research Use Only.