R&D Systems™ Recombinant Human/Mouse/Rat Activin A, ACFP Protein

Catalog No.	Quantity
ACFP338	50 μg

Catalog No. ACFP338 Supplier R&D Systems™ Supplier No. ACFP338

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL. The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626).

Specifications

• Accession Number: P08476
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in HCl.
• Gene ID (Entrez): 3624
• Molecular Weight (g/mol): M.W. (Observed): 13 kDa, reducing conditions; M.W. (theoretical): 13 kDa (monomer)
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived Activin A protein Met1-Ser426 Produced in an animal component free process (ACFP).
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: INHBA
• Specific Reactivity: Activin A
• Conjugate: Unconjugated
• Species: Human, Mouse, Rat
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Activin A