R&D Systems™ Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. ED50 for this effect is 0.08 to 0.48 µg/mL.

Manufacturer:  R&D Systems™ 355BEC100/CF

Catalog No. 35-5BE-C100CF

Specifications

• Species: Human, Mouse, Rat
• Accession Number: P12643
• Gene ID (Entrez): 650
• Name: BDA2, BMP2, BMP-2, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2, SSFSC
• Quantity: 100 µg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Buffer: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.

• Endotoxin Level: <0.01 EU per 1 µg of the protein by the LAL method.
• For Use With (Application): Bioactivity
• Molecular Weight: 12.8 kDa (monomer)
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Source: E. coli-derived BMP-2 protein Ala284-Arg396 This form is not detected using human BMP-2 Quantikine ELISA (Catalog # DBP200 ).
• Gene Symbol: BMP2
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCl.