R&D Systems™ Recombinant Human/Mouse/Rat BMP-2 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.

Specifications

• Accession Number: P12643
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween™ 80 and Glutamic Acid with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 μm filtered solution in Glycine, Sucrose, Tween™ 80 and Glutamic Acid with BSA as
• Gene ID (Entrez): 650
• Molecular Weight (g/mol): 13 kDa (monomer)
• Quantity: 100 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein Gln283-Arg396
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: BDA2, BMP2, BMP-2, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2, SSFSC
• Gene Symbol: BMP2
• Conjugate: Unconjugated
• Cross Reactivity: BMP-2
• Species: Human, Mouse, Rat
• Reconstitution: Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.