R&D Systems™ Recombinant Human/Mouse/Rat Irisin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human/Mouse/Rat Irisin Protein is derived from CHO. The Recombinant Human/Mouse/Rat Irisin Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q8NAU1
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 252995
• Molecular Weight (g/mol): Observed M.W.: 15-37kDa, reducing conditions, Theoretical M.W.: 13kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived Irisin/FNDC5 protein Asp32-Glu143,with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: fibronectin type III domain containing 5, fibronectin type III domain-containing protein 5, FNDC5, FRCP2, FRCP2Fibronectin type III repeat-containing protein 2
• Common Name: Irisin/FNDC5
• Gene Symbol: FNDC5
• Biological Activity: Measured by its ability to induce p38 MAPK activation in 3T3-L1 mouse embryonic fibroblast adipose-like cells. 1μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.
• Conjugate: Unconjugated
• Species: Human, Mouse, Rat
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Asp32-Glu143, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining