R&D Systems™ Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 μM.

Specifications

• Accession Number: P61081.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
• Gene ID (Entrez): 9040
• Molecular Weight (g/mol): M.W. (theoretical): 21 kDa
• Name: UBE2M/Ubc12
• Quantity: 100 μg
• Source: E. coli-derived UBE2M/Ubc12 protein Met1 - Lys183
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: UBE2M
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: UBE2M/Ubc12