R&D Systems™ Recombinant Human N-Acetylglucosaminyltransferase2/MGAT2

Description

HA-tag

Measured by its ability to modify the glycan Cy5-labeled M1N1f and thereby creating a band shift. Able to convert >50% of the substrate glycan M1N1f to the product glycan, as measured under the described conditions.

Specifications

• Accession Number: Q10469.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 4247
• Molecular Weight (g/mol): M.W.-theroretical: 48.7 kDa, M.W.-observed: 46-54 kDa, under reducing conditions
• Quantity: 25 μg
• Source: Human embryonic kidney cell, HEK293-derived human N-Acetylglucosaminyltransferase 2/MGAT2 protein Asn34-Gln447, with N-terminal HA tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Beta-1,2-N-acetylglucosaminyltransferase II, CDG2A, EC 2.4.1.143, GlcNAc-T II, GLCNACTII, GNT2, GNT-IICDGS2, Mannoside acetylglucosaminyltransferase 2, mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, UDP-N-acetylglucosamine:alpha-6-D-mannosidebeta-1,2-N-acetylglucosaminyltransferase II
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: MGAT2