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R&D Systems™ Recombinant Human N-Acetylglucosaminyltransferase2/MGAT2

Catalog No. 10711GT025
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25 μg
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10711GT025 25 μg
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HA-tag

Measured by its ability to modify the glycan Cy5-labeled M1N1f and thereby creating a band shift. Able to convert >50% of the substrate glycan M1N1f to the product glycan, as measured under the described conditions.

Specifications

Accession Number Q10469.1
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Gene ID (Entrez) 4247
Molecular Weight (g/mol) M.W.-theroretical: 48.7 kDa, M.W.-observed: 46-54 kDa, under reducing conditions
Quantity 25 μg
Source Human embryonic kidney cell, HEK293-derived human N-Acetylglucosaminyltransferase 2/MGAT2 protein Asn34-Gln447, with N-terminal HA tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Alias alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Beta-1,2-N-acetylglucosaminyltransferase II, CDG2A, EC 2.4.1.143, GlcNAc-T II, GLCNACTII, GNT2, GNT-IICDGS2, Mannoside acetylglucosaminyltransferase 2, mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, UDP-N-acetylglucosamine:alpha-6-D-mannosidebeta-1,2-N-acetylglucosaminyltransferase II
Conjugate Unconjugated
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein MGAT2
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