R&D Systems™ Recombinant Human Neurofascin Protein

Catalog No.	Quantity
82-08N-F050	50 μg

Catalog No. 82-08N-F050 Supplier R&D Systems™ Supplier No. 8208NF050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Neurofascin, immobilized at 6 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Specifications

• Accession Number: NP_055905.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 23114
• Molecular Weight (g/mol): M.W. (Observed): 120-140 kDa, reducing conditions; M.W. (theoretical): 116 kDa
• Name: Neurofascin
• Preparation Method: Reconstitute at 500μg/mL in sterile PBS.
• Quality Control Testing: SDS-PAGE: 120-140kDa, reducing conditions
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Neurofascin protein Ile25-Trp1039, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Termination: Ile25
• Without Additives: Carrier Protein
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: NFASC
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.
• Protein: Neurofascin