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R&D Systems™ Recombinant Human Neutrophil Elastase/ELA2 Protein

Catalog No. 9167SE020
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91-67S-E020 20 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

The Recombinant Human Neutrophil Elastase/ELA2 Protein is derived from CHO. The Recombinant Human Neutrophil Elastase/ELA2 Protein has been validated for the following applications: Enzyme Activity.

Specifications

Accession Number P08246
For Use With (Application) Enzyme Activity
Gene ID (Entrez) 1991
Molecular Weight (g/mol) Observed M.W.: 29-39kDa, reducing conditions, Theoretical M.W.: 25kDa
Quantity 20 μg
Source Chinese Hamster Ovary cell line,CHO-derived human Neutrophil Elastase/ELA2 protein Ser28-Asn252,with a C-terminal 10-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU per 1μg of the protein by the LAL method.
Gene Alias Bone marrow serine protease, EC 3.4.21, EC 3.4.21.37, ELA2, ELA2granulocyte-derived elastase, ELANE, elastase 2, neutrophil, elastase, neutrophil expressed, Elastase-2, GE, HLEelastase-2, HNE, Human leukocyte elastase, Leukocyte Elastase, medullasin, NE, neutrophil elastase, PMN elastase, PMN-E, polymorphonuclear elastase, SCN1
Common Name Neutrophil Elastase/ELA2
Biological Activity Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC). The specific activity is >1,500 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Chinese Hamster Ovary cell line, CHO-derived Ser28-Asn252, with a C-terminal 10-His tag
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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