R&D Systems™ Recombinant Human NRG1-beta 1/HRG1-beta 1 His Protein, CF

Description

His-tag

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.250-2.50 ng/mL.

Specifications

• Accession Number: NP_039250.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3084
• Molecular Weight (g/mol): M.W. (Observed): 36-49 kDa, under reducing conditions., M.W. (Theroretical): 26 kDa
• Quantity: 1 mg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Neuregulin-1 beta 1/NRG1 beta 1 protein Ser20-Lys246, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF, neuregulin 1, NRG1-IT2, SMDF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Neuregulin-1 beta 1/NRG1 beta 1