R&D Systems™ Recombinant Human NT-3 Protein

Description

(HEK293 expressed)

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 5.00-60.0 ng/mL.

Specifications

• Accession Number: P20783.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 4908
• Molecular Weight (g/mol): MolecularWeight-observed: 12-15 kDa, under reducing conditions., MolecularWeight-theroretical: 14 kDa
• Quantity: 100 μg
• Source: Human embryonic kidney cell, HEK293-derived human NT-3 protein Tyr139-Thr257
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: HDNF, MGC129711, Nerve growth factor 2, Neurotrophic factor, neurotrophin 3, neurotrophin-3, NGF2, NGF-2, NT3, NT-3, NTF3
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes (at 200 μg/mL in PBS.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: NT-3