R&D Systems™ Recombinant Human O-GlcNAcTransferase/OGT Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to transfer GlcNAc from UDP-GclNAc to peptide OGT substrate from AnaSpec, Inc. The specific activity is >25 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: O15294
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
• Gene ID (Entrez): 8473
• Molecular Weight (g/mol): M.W. (Observed): 70 kDa, reducing conditions; M.W. (theoretical): 81 kDa
• Name: O-GlcNAc Transferase/OGT
• Quality Control Testing: SDS-PAGE: 70kDa, reducing conditions
• Quantity: 10 μg
• Source: E. coli-derived human O-GlcNAc Transferase/OGT protein Cys323-Glu1041, with C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Regulatory Status: RUO
• Termination: Cys323
• Without Additives: Carrier Protein
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: EC 2.4.1, EC 2.4.1.255 FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGlcNAc Transferase, O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, OGT, O-linked N-acetylglucosamine (GlcNAc) transferase(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), O-linked N-acetylglucosamine transferase 110 kDa subunit, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDasubunit, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase
• Gene Symbol: OGT
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: O-GlcNAc Transferase p110 subunit