R&D Systems™ Recombinant Human OX40 Ligand/TNFSF4 Protein

Description

Recombinant Protein

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296. The ED50 for this effect is typically 1-5 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX ) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5-3 ng/mL.

Specifications

• Accession Number: Q6FGS4
• For Use With (Application): Bioactivity, Binding Activity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 7292
• Molecular Weight (g/mol): M.W.-observed: 28-34 kDa, reducing conditions, M.W.-theroretical: 18 kDa
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived human OX40 Ligand/TNFSF4 protein MHHHHHHHHHH GGGSGGGSGGGS IEGR Human OX40 Ligand (Gln51-Leu183) (N-terminus) Accession # Q6FGS4 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, gp34, O x 40 antigen ligand, O x 40 ligand, O x 40 L, OX-40 L, O x 4 OL, TAX transcriptionally-activated glycoprotein 1, tax-transcriptionally activated glycoprotein 1 (34 kD), tax-transcriptionally activated glycoprotein 1, 34 kD, TNFSF4, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4, TXGP1
• Gene Symbol: TNFSF4
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: OX40 Ligand/TNFSF4