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R&D Systems™ Recombinant Human PD-L1/B7-H1 Fc Alexa Fluor™ 488 Protein

Catalog No. AFG156020
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20 μg
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AFG156020 20 μg
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Fc Chimera

Measured by flow cytometry for its ability to bind anti-human PD-L1/B7-H1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human PD-L1/B7-H1 Fc Chimera Alexa Fluor™ 488 (Catalog # AFG156) that produces 50% of the binding response is 15.0-150 ng/mL.

Specifications

Accession Number Q9NZQ7.1
For Use With (Application) Bioactivity
Formulation Supplied as a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Gene ID (Entrez) 29126
Molecular Weight (g/mol) MolecularWeight-observed: 70-75 kDa, under reducing conditions., MolecularWeight-theroretical: 52 kDa (monomer)
Quantity 20 μg
Source Mouse myeloma cell line, NS0-derived human PD-L1/B7-H1 protein Human PD-L1 (Phe19-Thr239) (N-terminus) Accession # Q9NZQ7.1 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Alias Avelumab, B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
Conjugate Alexa Fluor 488
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PD-L1
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